LOCUS       40924_42009       10231 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pSZPT1 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10231)
  AUTHORS   Kanda K, Ishida T, Hirota R, Ono S, Motomura K, Ikeda T, Kitamura K,
            Kuroda A.
  TITLE     Application of a phosphite dehydrogenase gene as a novel dominant 
            selection marker for yeasts
  JOURNAL   J. Biotechnol. 182-183, 68-73 (2014)
  PUBMED    24786825
REFERENCE   2  (bases 1 to 10231)
  AUTHORS   Hirota R.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2013) Contact:Ryuichi Hirota Hiroshima University,
            Department of Biotechnology; 1-3-1, Higashi-Hiroshima, Hiroshima 
            739-8530, Japan
REFERENCE   3  (bases 1 to 10231)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10231)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol. 182-183, 68-73 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-NOV-2013) Contact:Ryuichi Hirota Hiroshima University, 
            Department of Biotechnology; 1-3-1, Higashi-Hiroshima, Hiroshima 
            739-8530, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1251..2261
                     /strain="4506"
                     /mol_type="other DNA"
                     /country="Japan"
                     /isolation_source="soil"
                     /collected_by="Hirota,R."
                     /collection_date=2011
                     /db_xref="taxon:1094341"
                     /organism="Ralstonia sp. 4506"
     misc_feature    7..12
                     /label=SphI recognition site
                     /note="SphI recognition site"
     misc_feature    13..18
                     /label=PstI recognition site
                     /note="PstI recognition site"
     promoter        49..1204
                     /label=nmt1 promoter
                     /note="wild-type nmt1 promoter from Schizosaccharomyces
                     pombe, conferring strong thiamine-repressible expression"
     misc_feature    1211..1216
                     /label=SalI recognition site
                     /note="SalI recognition site"
     CDS             1233..1250
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             1251..2261
                     /codon_start=1
                     /gene="RsptxD"
                     /product="phosphite dehydrogenase"
                     /label=RsptxD
                     /protein_id="BAP74302.1"
                     /translation="MKPKVVLTHWVHPEIIELLSASADVIPNTTRETLPRSEVIARAKD
                     ADALMAFMPDSIDSAFLEECPKLRVIGAALKGYDNFDVNACTRHGVWLTIVPDLLTIPT
                     AELTIGLLLGLTRHMLEGDRQIRSGHFQGWRPTLYGSGLTGKTLGIIGMGAVGRAIAQR
                     LAGFEMNLLYCDPIPLNAEQEKAWHVQRVTLDELLEKCDYVVPMVPMAAETLHLIDATA
                     LAKMKTGSYLINACRGSVVDENAVIAALASGKLAGYAADVFEMEEWIRADRPQAIPKAL
                     LDNTAQTFFTPHLGSAVKEVRLEIERQAAMNIIQALAGEKPMGAINQPYPGVKAA"
     gene            1251..2261
                     /gene="RsptxD"
                     /label=RsptxD
     CDS             2262..2285
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             2292..2315
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     terminator      2367..2554
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    2584..2589
                     /label=SpeI recognition site
                     /note="SpeI recognition site"
     misc_feature    2590..3816
                     /label=leu1-5'
                     /note="leu1-5'"
     CDS             complement(4221..5012)
                     /label=S. pombe ura4
                     /note="orotidine 5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     misc_feature    5548..5553
                     /label=EcoRI recognition site
                     /note="EcoRI recognition site"
     rep_origin      complement(5621..6404)
                     /direction=LEFT
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     misc_feature    6761..7114
                     /label=leu1-3'
                     /note="leu1-3'"
     misc_feature    7115..7120
                     /label=SacI recognition site
                     /note="SacI recognition site"
     misc_feature    7121..7126
                     /label=EcoRI recognition site
                     /note="EcoRI recognition site"
     primer_bind     complement(7127..7143)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7356..7811
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8093..8197
                     /label=AmpR promoter
     CDS             8198..9055
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      9229..9817
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    10105..10126
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10141..10171
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10179..10195
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10203..10219
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgatcgactc tagaggatca gaaaattatc gccataaaag
       61 acagaataag tcatcagcgg ttgtttcatt tcctatattt tttttttatt tttttatttt
      121 ttaataaggg aaaatttaac gtctaaggat acagaagatt gttagcacat taaagtaata
      181 aaggcttaag tagtaagtgc cttagcatgt tattgtattt caaaggacat aatctaaaat
      241 aataacaata tcatttctca caagttattc aattttcttt tttttttcta ataatatcaa
      301 gaatgtatta tttgtttgac ataagtcaac taatttattt aatatgctgg attaatcttg
      361 cagacatgta aattaacaag ttttagtcaa ataacgttga agtttcaatg aactcaaata
      421 atttctcttt ttttttatat aactctaatc tgatttatat tttccgcagg gatcaactga
      481 agttatgaca tttggattgg atcacttata accttggtcg ccaaataata caaaaatcag
      541 cgttataaaa caaagaaggt ttttgttaag aaattaatcc tctttcttga taagaaagtt
      601 gaaccgaaat tgcagatact gatatatgaa aataataccc acaattttgg gaatagcgca
      661 agcctcaatt taaacaatag gtgaggacac atgataatga cctcaatgat tgttagaaga
      721 aaagagcctc attacaaaat cgaaaaatga atggttgggt acaagtttcc aaaacatggt
      781 aaagtggact ttgcgtatga gacgtaaata gaaaaaaaca cttgttatat gttttctaga
      841 attattgttg tctctttatg gttggatgat gcaaaatagt aatttcggtt agttgctgta
      901 aaacaccacg agacaaatag atatggatat ttattaaatc aggaaaaacg taactctcgg
      961 ctactggatg gttcagtcac ccaacgatta ctggggagag aaaacagggc aaaagcaaag
     1021 cttaaaggaa tccgattgtc attcggcaat gtgcagcgaa actaaaaacc ggataatgga
     1081 cctgttaatc gaaacattga agatatataa aggaagagga atcctggcat atcatcaatt
     1141 gaataagttg aattaattat ttcaatctca ttctcacttt ctgacttata gtcgctttgt
     1201 taaagctagc gtcgacaggc ctggatccta tgcatcacca ccatcatcat atgaagccca
     1261 aagtcgtcct cacccactgg gtgcacccgg aaatcatcga attgttgtcc gctagcgccg
     1321 atgttatccc caacaccaca cgggaaacct tgccgcgttc tgaggtaatt gcgcgagcca
     1381 aagatgcgga tgcactcatg gctttcatgc cggacagcat cgacagcgcg tttctcgagg
     1441 aatgtccaaa gctgcgtgtc atcggcgccg cgcttaaagg ctatgataac ttcgatgtca
     1501 acgcctgcac acgccacggt gtatggctta cgattgtgcc ggatttgctt acgatcccga
     1561 ccgctgaact gactatcggc cttcttctcg gtttgacaag gcatatgctg gaaggcgata
     1621 ggcaaatccg tagcggacac ttccaaggct ggcggccgac actatatggc tctggtttga
     1681 caggaaaaac gcttggcatc attggtatgg gggcggtcgg ccgtgcaatc gcccagcgct
     1741 tggctggctt tgaaatgaat ctcttgtatt gcgatccgat tccgctcaat gccgaacaag
     1801 aaaaggcttg gcacgtacag cgcgtcacgc tcgatgaact gctcgaaaaa tgtgattatg
     1861 tcgtgccgat ggttccgatg gccgcagaga cactgcatct gatcgatgcc accgcgttgg
     1921 ccaagatgaa aaccggtagc tacctgatca atgcatgtcg cggctcggtc gtggatgaga
     1981 atgcggtgat agcagcactg gcgtctggaa aactagctgg atatgcagcc gatgtcttcg
     2041 agatggaaga atggatacgc gctgatcgcc cgcaggctat ccccaaggcg ctgctcgaca
     2101 atacggcaca aacgtttttt acgccgcatt tgggatcggc ggtcaaggaa gttcggcttg
     2161 aaatcgagcg gcaggcagcg atgaacatca tccaggcact cgctggtgaa aaaccgatgg
     2221 gcgcgattaa tcagccgtat ccgggagtaa aggcggcgtg agattataag gatgatgacg
     2281 ataaacccat ggactacaag gacgacgatg acaaagatat catgagatct cgactctaga
     2341 ggatccggcg cgccacttct aaataagcga atttcttatg atttatgatt tttattatta
     2401 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
     2461 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt
     2521 atgaggtcgc tcttattgac cacacctcta ccggcagatc cgctagggat aacagggtaa
     2581 tatactagta agatgattct tttatcgtcg ataacgatga ggatgtagac gattatgatg
     2641 agagtgatta gcttctaaaa tttggtgttt tcaaacgcta tttacagtac aatctgaaaa
     2701 aattggatga acattgtaaa tggtaggata cgagttccaa ttaatgtatt ttttaagtat
     2761 tacacttagc ttattaattt tatatataca ttctacctct gtgggtttaa agacaactcg
     2821 tacacatact caaattattt atgaacatta taatgcatat tcgtactgtc tattaaacat
     2881 ttttttgaca ttaaaaattt cgtttactaa cgtagaaagc gctgataatt gaggctacta
     2941 ttttaatatc tagaaacaaa gattcattaa ttaattaagt aagctccagg atacttgtat
     3001 atttcgttaa aaaagcgcaa tttcaacaat tcctatgaac atccatgatt tcatcacaaa
     3061 atcaaacata tcaggattta acgtgaatta tttgctttct tgtgattttc atctgatttc
     3121 tggtcattta cgttactgta aacaagcttt ttaaaaaaag gattttttac gcaatattat
     3181 cgatatccca atctgtagta taaaaagtgc aacagtgttg agtttcccga aaccaggcat
     3241 ttttgcggta gggtctgaaa ctttcacgaa accatttcac aacaaaactt cttcaacaac
     3301 atgtgtgcaa agaaaatcgt cgtcttacca ggagaccata ttggccctga aattgttgct
     3361 tctgccttgg aggttttgaa agtcgttgag aagaagcgac ctgagttaaa actcgagttt
     3421 gaagaacaca agattggagg tgcctctatt gatgcctatg gaaccccttt gactgacgag
     3481 actgtgaagg cttgtttgga agctgacggt gttcttttgg gtgccgttgg tggtcctgaa
     3541 tggaccaacc ccaattgtcg tcctgagcaa ggtttattga agcttcgtaa gagtatgggt
     3601 gtttgggcca accttcgacc ttgcaacttt gccagcaagt ctttagtcaa gtacagccct
     3661 ttgaagcctg aaatcgttga aggtgtcgat ttttgtgttg tacgagaact tactggaggt
     3721 tgttactttg gtgagcgcac tgaggacaac ggatcgggtt atgctatgga cacttggcct
     3781 tacagtttgg aagaagtttc tcgtattgct cgtttggcgg ccgcgggccc aacaccaatg
     3841 tttataacca agttttatct tgtttgtcta catggtattt tacattcatc tacatacatc
     3901 tttcattggc tttgtacata gttatcatta caagtctaaa aaaattcact cttttcttat
     3961 tcaatgtcaa tccaagagaa aagattgtgg taatgttgta ggagcatgtt taataaatta
     4021 ctatagcaaa ttacttttta ttcccaaggt gtttatctat aatagttaat attttagtcg
     4081 ctacataaaa ttttaccaaa gagtacttgt atactaattc taaatgcctt ctgacataaa
     4141 acgcctagga aaacaaacgc aaacaaggca tcgacttttt caataaccaa ccaaaaaaat
     4201 tttacattag tcttttttta atgctgagaa agtctttgct gatatgcctt ccaaccagct
     4261 tctctatatc tcttggcttc gacaacagga ttacgaccag ctccatagac tccacgacca
     4321 acaatgatga tgtcgctacc gcagtttaca atcacttctt caggagtacg atattgctgt
     4381 cccagcccgt ctcctttaac atccaagccg ataccagggg acatagttat gtagtcgctt
     4441 tgaaggttag gaaatcgacg accagctata aagccaaagc aaaaatcggt atgcttctca
     4501 aaccattcta aggttttctc tgtgtaggaa ccagtagcca aagagccttt ggaagacatt
     4561 tcagccaaaa gcaagagacc acgtcccaaa ggtaaaccaa cttctttgag gccttgtata
     4621 ataccctcgc ctggcactgt atggcaattt gtgatatgag cccaagaagc aattttgtac
     4681 acaccagatg catattgtag cttgacggta tttccaatgt ctgcgaattt gcgatcctca
     4741 aagataagaa aacgatgctt tttacctaag gccaccagtt tttctaccat atcctggtcg
     4801 aaatcctcga caacgtcaat atgtgtcttg ataacacaga catagggtcc aattttatct
     4861 accaattcta agatttcgga tttcttcgtc aaatcgaccg cgactgacaa gttgctttgc
     4921 ttttcttcca tcaaagccaa caattccttg gcaatgggat ttttcatccc ctcagctcta
     4981 gctgaatagc tttgaaatac tctagcatcc ataactttgc ttttaaacct ttaatttcga
     5041 tccaagcaaa aaagaggttc ttggtaggac aatacggtaa gaaaacacga catgtgcaga
     5101 gatgccgacg aagcatagtt aaactgggat ggtaaaatca attaagaatt tataaagaca
     5161 aaattgtata agtctctaaa acatcttaat tatacctcac agaactatct aaaatatatt
     5221 cacaaagtgc aaacattatc atgaaaaaga accattttaa tttaaagcaa gggcattaag
     5281 gcttatttac agaatttctt acttttgtaa agattataag gctgattatc tttttcacca
     5341 tgccaaaaat tacacaagat agaatggatg tttgaaatta aacgtgagta tacaaacaaa
     5401 tacactaggt aaatcgaaac atttttttct ccattaagta acaaattcct atttagagaa
     5461 agaatgctga gtagattaaa taatctatac aaactttttt aacacaaatg catacatata
     5521 gccagtggga tttgtagcta agcttccgaa ttcgagtcta actccttaac cactcggaca
     5581 tagtgactta tctgacactc attgtaaatt aatatatata taggcatttt gtttagttaa
     5641 aggtacttaa gtaattagta taaacgaacc aattttataa tcaggaagtt aagtgaatgg
     5701 tagcacatgt cgtaaaaatt gtgaattttt attgaataat attttaaata caagcctttc
     5761 tagactaggt atactcataa acatatatga gcaaaaggat agaggagatt acattgcatc
     5821 ttctacaaat ttatttattg ccctttactg aaaaattaaa taatgagtac taaatgataa
     5881 aaagcgctca gtacaagaaa gattgcaaaa atattgcatt cttcatgaat taaagttgca
     5941 tataaggcat attgaaagta atagtactaa aacagcagtt agcgaaaatt aatagaatta
     6001 tattcgcaag acaattgtga cattaaatta aaaaattgta gaatttttac tatcctcttt
     6061 aacgccatga gcctttataa aaaggttaaa ttagttttaa cattcttttt ttgagtaaga
     6121 gtttacaatt tatcaaaacc tgtgttatta tattcattag tttcaattta ttagcatcta
     6181 gagaaaaatc aattggcagt tacattgttg gaatttatga agaaaaagaa tctacaacgg
     6241 agaataagtt gctgatcgct ttccctaaaa ttgtatattt tgctgagctt attttgacat
     6301 ttcgttgaag ttttctctaa ttcgcattca ttttaagtaa aacaatgaga aataaaatta
     6361 caaaaaatac aaattaaaat acaattttta gctataatta tagacgatgc ccttgtatcc
     6421 cattctgtct cgcttgcccc tactttttat cttttatata ccataatgaa cgctgccgct
     6481 actaaccata ccccgatttt acatttcgga ctcccaagga cgtacaaaat agaaaactat
     6541 agaaaaaaat aatcagaaaa tagcatgtca tctctttgta aaacgcgttt gcaagaagaa
     6601 aggaaacaat ggagaagaga tcatccattt gtatgtaaat tttagtaaac ttgaagaaat
     6661 cactaacaac ttctcttact tagggattct atgcaaaacc ttgtaaatca tctgatggag
     6721 gactcgattt aatgaattgg aaggttgggc ccgcggccgc ttagctgaaa cttccaaccc
     6781 tcctgctccc gtcacattac tcgacaaagc taatgttttg gcaacttctc gtctttggcg
     6841 caagactgta gctaagattt ttaaggagga atatcctcac cttaccttaa aaaaccagct
     6901 cattgactcc gcagccatgc ttttggtcaa gagccctcgt acacttaacg gtgttgtttt
     6961 gactgacaac ttgtttggtg acattatttc agatgaggct tctgtcattc ctggtagctt
     7021 gggccttttg ccttctgcct ccctttccgg tgtggtagga aaatcagaag aaaaggttca
     7081 ttgtttggtt gagcccattc acggtagcgc tcccgagctc gaattcactg gccgtcgttt
     7141 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     7201 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     7261 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     7321 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     7381 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     7441 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     7501 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     7561 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     7621 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     7681 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     7741 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     7801 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     7861 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     7921 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     7981 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     8041 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     8101 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     8161 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     8221 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     8281 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     8341 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     8401 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     8461 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     8521 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8581 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8641 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8701 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8761 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8821 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8881 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8941 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     9001 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     9061 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     9121 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     9181 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     9241 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     9301 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     9361 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9421 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9481 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9541 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9601 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9661 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9721 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9781 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9841 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9901 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9961 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
    10021 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
    10081 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    10141 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    10201 cacaggaaac agctatgacc atgattacgc c
//
